A GABARAP-PtdIns3K-C1 positive feedback loop at the heart of the phagophore nucleation.
Dessus AN, Ohashi Y, Bourguet M, Morgan TE, Nunez A, Manifava M, Ktistakis NT, Williams RL
Nat Commun. 2026 Jul 30;17(1):9473. doi: 10.1038/s41467-026-76135-w.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 252672 | pYO1562 | Expression of mCherry-Beclin1 in mammalian cells |
| 252673 | pYO1601 | Protein expression of 6xHis-TEV-mCerulean-HsLC3A (1-120) in bacteria |
| 252674 | pYO1609 | Protein expression of 6xHis-TEV-mCerulean-HsLC3B (1-120) in bacteria |
| 252675 | pYO1610 | Protein expression of 6xHis-TEV-mCerulean-HsLC3C (1-126) in bacteria |
| 252676 | pYO1611 | Protein expression of 6xHis-TEV-mCerulean HsGABARAP (1-116) in bacteria |
| 252677 | pYO1612 | Protein expression of 6xHis-TEV-mCerulean-HsGABARAPL1 (1-116) in bacteria |
| 252678 | pYO1613 | Protein expression of 6xHis-TEV-mCerulean-HsGABARAPL2 (1-116) in bacteria |
| 252679 | pYO1594 | Protein expression of mCerulean-TEV-14xHis |
| 252680 | pYO1495 | Protein expression of mCherry-TEV-GST |
| 252681 | pYO1499 | Protein expression of ZZ-3xTEV-mATG7 |
| 252682 | pYO1928 | Expression of ZZ-TEV-HsBeclin1 in yeast |
| 252683 | pYO1932 | Expression of ZZ-3xTEV-HsATG14L-mCherry in yeast |
| 252684 | pYO1933 | Expression of ZZ-3xTEV-HsATG14L (1-434)-mCherry in yeast |
| 252685 | pYO1943 | Expression of ZZ-TEV-HsBeclin1 (F97A I100A) in yeast |
| 252686 | pYO1948 | Expression of ZZ-3xTEV-HsATG14L (1-434 ΔYVAV)-mCherry in yeast |
| 252687 | pYO1797 | Expression of HsATG14L (W435A L438A) in mammalian cells |
| 252688 | pYO1845 | Expression of HsBeclin1 (F97A I100A) in mammalian cells |
| 252689 | pYO1982 | Expression of HsBeclin1 (F97A I100A) and HsATG14L (ΔYVAV) in mammalian cells |
| 252690 | ADp1 | Protein expression of GST-TEV-HsGABARAP (1-116) in bacteria |
| 252691 | ADp2 | Protein expression of 6xHis-TEV-HsGABARAP (1-116) in bacteria |
| 252692 | ADp3 | Protein expression of GST-TEV-HsGABARAPL2 (1-116) in bacteria |
| 252693 | ADp4 | Protein expression of 6xHis-TEV-HsGABARAPL2 (1-116) in bacteria |
| 252694 | ADp5 | Protein expression of GST-TEV-HsGABARAPL1 (1-116) in bacteria |
| 252695 | ADp6 | Protein expression of 6xHis-TEV-HsGABARAPL1 (1-116) in bacteria |
| 252696 | ADp7 | Protein expression of GST-TEV-HsLC3A (1-120) in bacteria |
| 252697 | ADp8 | Protein expression of 6xHis-TEV-HsLC3A (1-120) in bacteria |
| 252698 | ADp9 | Protein expression of GST-TEV-HsLC3B (1-120) in bacteria |
| 252699 | ADp10 | Protein expression of 6xHis-TEV-HsLC3B (1-120) in bacteria |
| 252700 | pYO1595 | Protein expression of 6xHis-TEV-HsLC3C (1-126) in bacteria |
| 252701 | pYO1596 | Protein expression of GST-TEV-HsLC3C (1-126) in bacteria |
| 252702 | ADp17 | Protein expression of GST-tev-GABARAP_L117C in bacteria |
| 252703 | ADp19 | Protein expression of 6xHis-TEV-mCerulean-GABARAP_Y115S_L117C in bacteria |