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pYO1943
(Plasmid #252685)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252685 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pRS424
  • Vector type
    Yeast Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    ZZ-TEV-HsBeclin1 (F97A I100A)
  • Species
    H. sapiens (human)
  • Mutation
    F97A, I100A
  • Entrez Gene
    BECN1 (a.k.a. ATG6, VPS30, beclin1)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Unspecified (unknown if destroyed)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pYO1943 was a gift from Roger Williams (Addgene plasmid # 252685 ; http://n2t.net/addgene:252685 ; RRID:Addgene_252685)

  • For your References section:

    A GABARAP-PtdIns3K-C1 positive feedback loop at the heart of the phagophore nucleation. Dessus AN, Ohashi Y, Bourguet M, Morgan TE, Nunez A, Manifava M, Ktistakis NT, Williams RL. Nat Commun. 2026 Jul 30;17(1):9473. doi: 10.1038/s41467-026-76135-w. 10.1038/s41467-026-76135-w PubMed 42693081
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