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pJB040 pAAV EF1a Nuc-flox(mCherry)-mNeonGreen
(Plasmid #252772)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 252772 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pOTTC407
  • Backbone manufacturer
    NIDA OTTC
  • Vector type
    AAV

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Nuc-flox(mCherry)-mNeonGreen
  • Species
    Synthetic
  • Promoter hEF1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site KpnI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer N/A
  • 3′ sequencing primer N/A
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Leonard Lab plasmid reference number: L5931 . Please visit https://doi.org/10.1101/2025.07.02.662894 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJB040 pAAV EF1a Nuc-flox(mCherry)-mNeonGreen was a gift from Joshua Leonard (Addgene plasmid # 252772 ; http://n2t.net/addgene:252772 ; RRID:Addgene_252772)

  • For your References section:

    Distinguishing Pseudotransduction and True Transduction Enables Characterization and Bioengineering of Extracellular Vesicle-Adeno-Associated Virus Vectors. Boucher JD, Stranford DM, Edelstein HI, Tullman-Ercek D, Kamat NP, Leonard JN. J Extracell Vesicles. 2026 Apr;15(4):e70258. doi: 10.1002/jev2.70258. 10.1002/jev2.70258 PubMed 41942279
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