pJB041 (pAAV EF1a flox(mCherry)-mNeonGreen
(Plasmid #252773)

• Purpose: An AAV vector that expresses a Cre-dependent mCherry to mNeonGreen
• Depositing Lab: Joshua Leonard
• Publication: Boucher et al J Extracell Vesicles. 2026 Apr;15(4):e70258. doi: 10.1002/jev2.70258.

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Backbone

• Vector backbone: pOTTC407
• Backbone manufacturer: NIDA OTTC
• Vector type: AAV

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: flox(mCherry)-mNeonGreen
• Species: Synthetic
• Promoter: hEF1a

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: KpnI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: N/A
• 3′ sequencing primer: N/A

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Leonard Lab plasmid reference number: L5932 . Please visit https://doi.org/10.1101/2025.07.02.662894 for bioRxiv preprint.