c-cre[LVA]-loxPP-syfp2
(Bacterial strain #252782)

• Purpose: Chromosomally integrated, cumate-inducible Cre-lox reporter for low-rate recombination and fluorescent readout in E. coli.
• Depositing Lab: Andrea Giometto
• Publication: Garabello et al bioRxiv [Preprint]. 2025 Sep 2:2024.10.02.616356. doi: 10.1101/2024.10.02.616356.

Backbone

• Vector backbone: n/a
• Vector type: This is a strain, not a plasmid

Growth in Bacteria

• Bacterial Resistance(s): Chloramphenicol and Kanamycin, 5 & 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): c-cre[LVA]-loxPP-syfp2
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: sAJM.1504; cymRAM // attTn7::PcymRC-cre[LVA], T - PrpsL - loxP - T - loxP - syfp2
• Species: n/a

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Strain can be grown in LB with 50 μg/mL Kanamycin, or without antibiotics. Parental strain is the marionette sensor-Clo sAJM.1504, with background DH10B.

Colonies should be checked by fluorescence microscopy for YFP expression before selecting one for experiments. Although the likelihood of picking a colony that underwent recombination without inducer is low (~1/100), it is still possible and could lead to downstream experimental issues.