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Ska Ska3 T358/360A
(Plasmid #253355)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253355 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pBIG1a
  • Backbone size w/o insert (bp) 5915
  • Vector type
    Insect Expression
  • Selectable markers
    Gentamicin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin and Spectinomycin, 100 & 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ska3
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1236
  • Mutation
    T358A/T360A
  • Entrez Gene
    SKA3 (a.k.a. C13orf3, RAMA1)
  • Promoter polyhedrin promoter

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Ska1 (765bp 6xHis ID:220134), Ska2 (363bp ID:348235). Please visit https://doi.org/10.1101/2025.07.06.663352 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Ska Ska3 T358/360A was a gift from Vladimir Volkov (Addgene plasmid # 253355 ; http://n2t.net/addgene:253355 ; RRID:Addgene_253355)

  • For your References section:

    Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes. Radhakrishnan RM, Stokes L, Day M, Huis in ’t Veld PJ, Volkov VA. EMBO J (2026). 10.1038/s44318-026-00749-5
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