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Microtubule end stabilisation by cooperative oligomers of Ska and Ndc80 complexes

Radhakrishnan RM, Stokes L, Day M, Huis in ’t Veld PJ, Volkov VA
EMBO J (2026). (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
253353Ska Ska1 R236APlasmid for the expression and purification of Ska1 R236A, Ska2, and Ska3 protein subunit complexes in insect cells
253354Ska Ska3 ΔCPlasmid for the expression and purification of Ska1, Ska2, and Ska3 ΔC protein subunit complexes in insect cells
253355Ska Ska3 T358/360APlasmid for the expression and purification of Ska1 T358A/T360A, Ska2, and Ska3 protein subunit complexes in insect cells
253357GFP-Ska1Plasmid for the expression of GFP-Ska1 in mammalian cells
253358GFP-Ska1 R236APlasmid for the expression of GFP-Ska1 in mammalian cells
253359Ska1 MTBDPlasmid for the expression of Ska1 microtubule binding domain in bacteria
253360Ska1 LMTBDPlasmid for the expression of Ska1 linker and microtubule binding domain in bacteria
253363Ska1 LZ-LMTBD R155APlasmid for the expression of mutant Ska1 microtubule binding domain, with N-terminal leucine zipper, in bacteria
253364Ska1 LZ-LMTBD K183/184APlasmid for the expression of mutant Ska1 microtubule binding domain, with N-terminal leucine zipper, in bacteria
253365Ska1 LZ-LMTBD K203/206APlasmid for the expression of mutant Ska1 microtubule binding domain, with N-terminal leucine zipper, in bacteria
253366Ska1 LZ-LMTBD K223/226APlasmid for the expression of mutant Ska1 microtubule binding domain, with N-terminal leucine zipper, in bacteria
253367Ska1 LZ-LMTBD R236APlasmid for the expression of mutant Ska1 microtubule binding domain, with N-terminal leucine zipper, in bacteria
253368Ska1 LZ-LMTBD R245APlasmid for the expression of mutant Ska1 microtubule binding domain, with N-terminal leucine zipper, in bacteria

Antibodies from Article