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pB_hU6_Cas9-t2a-Puro
(Plasmid #253414)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253414 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    piggyBac
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cas9
  • Species
    Synthetic
  • Promoter Cbh
  • Tags / Fusion Proteins
    • 3xFLAG (N terminal on insert)
    • 2a-Puro (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The guide sequence can be cloned into this plasmid using BbsI(BpiI) sites with a goldengate reaction.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pB_hU6_Cas9-t2a-Puro was a gift from Joanna Wysocka (Addgene plasmid # 253414 ; http://n2t.net/addgene:253414 ; RRID:Addgene_253414)

  • For your References section:

    Promoter strength and position govern promoter competition through transcript-dependent insulation. Koska M, Nagano M, Swigut T, Boettiger AN, Hansen AS, Wysocka J. Nat Genet. 2026 Jul 20. doi: 10.1038/s41588-026-02691-y. 10.1038/s41588-026-02691-y PubMed 42477113
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