Promoter strength and position govern promoter competition through transcript-dependent insulation.
Koska M, Nagano M, Swigut T, Boettiger AN, Hansen AS, Wysocka J
Nat Genet. 2026 Jul 20. doi: 10.1038/s41588-026-02691-y.
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253414 | pB_hU6_Cas9-t2a-Puro | piggyBac integration and stable expression of sgRNA and SpCas9. Based on plasmid px459 (Addgene #62988) |
| 253415 | pB_hU6_dCas9-t2a-Puro | piggyBac integration and stable expression of guide and dCas9. |
| 253416 | CARGO_Ef1a-6mer | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Ef1a promoter |
| 253417 | CARGO_mTagBFP2-6mer | 6-mer gRNA array targeting Sp dCas9/Cas9 to the mTagBFP2 transgene with intron |
| 253418 | CARGO_Neutral-6mer | 6-mer gRNA array targeting Sp dCas9/Cas9 to the Neutral promoter (Ecoli uraA CDS) |
| 253419 | pGEM-T-hU6-spSL-CARGOConstant | Empty backbone with CARGO constant region |
| 253420 | pK335_CARGO-NoReporter | Backbone for CARGO cloning, with U6 promoter, gRNA scaffold and Kanamycin resistence, without flurophore |
| 253421 | Rad21-C-term-dTagPuro | C-terminal tagging of mouse Rad21 with FKBP and Puromycin |
| 253422 | SCR-CTCFmutated | introduces CTCF mutations and a selection casette into the core SCR |
| 253423 | pDonorRMCE-For-FRT-MCS-mTagBFP2wintron-SV40pA-FRT3 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with MCS for promoter integrations |
| 253424 | pDonorRMCE-Rev-FRT3-MCS-mTagBFP2wintron-SV40pA-FRT | Donor plasmid for FLP/FRT mediated recombination into landing pad in the reverse direction with MCS for promoter integrations |
| 253425 | pDonorRMCE-For-FRT-MCS-mTagBFP2wintron-noSV40pA-FRT3 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with MCS for promoter integrations, without an SV40 polyA signal after reporter |
| 253426 | pDonorRMCE-Rev-FRT3-MCS-mTagBFP2wintron-noSV40pA-FRT | Donor plasmid for FLP/FRT mediated recombination into landing pad in the reverse direction with MCS for promoter integrations, without an SV40 polyA signal after reporter |
| 253427 | pDonorRMCE-For-Rpl13a-CTCFDeleted-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with Rpl13a promoter (CTCF sites are deleted) |
| 253428 | pDonorRMCE-For-Rpl13a-CTCFScrambled-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with Rpl13a promoter (CTCF sites are mutated) |
| 253429 | pDonorRMCE-For-dBx-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dBx |
| 253430 | pDonorRMCE-For-dPst-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dPst |
| 253431 | pDonorRMCE-For-dSac-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dSac |
| 253432 | pDonorRMCE-For-dP2-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dP2 |
| 253433 | pDonorRMCE-For-dP4-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dP4 |
| 253434 | pDonorRMCE-For-dl1-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl1 |
| 253435 | pDonorRMCE-For-dl2-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl2 |
| 253436 | pDonorRMCE-For-dl3-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl3 |
| 253437 | pDonorRMCE-For-dl6-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl6 |
| 253438 | pDonorRMCE-For-dl8-mTagBFP2 | Donor plasmid for FLP/FRT mediated recombination into landing pad in the forward direction with mutant Ef1a promoter - dl8 |
| 253439 | pDonorRMCE-For-Ef1a-mTagBFP2-luciferase | Modified donor plasmid for FLP/FRT RMCE in forward direction with longer reporter (mTagBFP2+luciferase) and Ef1a promoter |
| 253440 | pDonorRMCE-Rev-Ef1a-mTagBFP2-luciferase | Modified donor plasmid for FLP/FRT RMCE in reverse direction with longer reporter (mTagBFP2+luciferase) and Ef1a promoter |
| 253441 | pDonorRMCE-For-Neutral-mTagBFP2-luciferase | Modified donor plasmid for FLP/FRT RMCE in forward direction with longer reporter (mTagBFP2+luciferase) and Neutral promoter |
| 253442 | pDonorRMCE-Rev-Neutral-mTagBFP2-luciferase | Modified donor plasmid for FLP/FRT RMCE in reverse direction with longer reporter (mTagBFP2+luciferase) and Neutral promoter |