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pDonorRMCE-Rev-FRT3-MCS-mTagBFP2wintron-SV40pA-FRT
(Plasmid #253424)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253424 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pGL3
  • Backbone manufacturer
    Promega
  • Vector type
    Mammalian Expression, Mouse Targeting ; FLP/FRT
  • Tag / Fusion Protein
    • mTagBFP2 with intron (N terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    None
  • Tag / Fusion Protein
    • mTagBFP2 with intron (N terminal on backbone)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

5' cloning site: KpnI (not destroyed), 3' cloning site: MfeI (not destroyed)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonorRMCE-Rev-FRT3-MCS-mTagBFP2wintron-SV40pA-FRT was a gift from Joanna Wysocka (Addgene plasmid # 253424 ; http://n2t.net/addgene:253424 ; RRID:Addgene_253424)

  • For your References section:

    Promoter strength and position govern promoter competition through transcript-dependent insulation. Koska M, Nagano M, Swigut T, Boettiger AN, Hansen AS, Wysocka J. Nat Genet. 2026 Jul 20. doi: 10.1038/s41588-026-02691-y. 10.1038/s41588-026-02691-y PubMed 42477113
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