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pDonorRMCE-Rev-Ef1a-mTagBFP2-luciferase
(Plasmid #253440)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253440 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDonorRMCE-Rev-FRT-MCS-mTagBFP2wintron-SV40pA-FRT3
  • Vector type
    Mammalian Expression, Mouse Targeting ; FLP/FRT
  • Selectable markers
    mTagBFP2

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTagBFP2-t2a-luciferase
  • Species
    Synthetic
  • Promoter Ef1a

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SbfI (not destroyed)
  • 3′ cloning site PacI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pDonorRMCE-Rev-Ef1a-mTagBFP2-luciferase was a gift from Joanna Wysocka (Addgene plasmid # 253440 ; http://n2t.net/addgene:253440 ; RRID:Addgene_253440)

  • For your References section:

    Promoter strength and position govern promoter competition through transcript-dependent insulation. Koska M, Nagano M, Swigut T, Boettiger AN, Hansen AS, Wysocka J. Nat Genet. 2026 Jul 20. doi: 10.1038/s41588-026-02691-y. 10.1038/s41588-026-02691-y PubMed 42477113
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