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CARGO_mTagBFP2-6mer
(Plasmid #253417)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253417 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pK335
  • Vector type
    Mammalian Expression, CRISPR
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Array of guide RNAs
  • gRNA/shRNA sequence
    Unspecified
  • Species
    Synthetic

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CARGO_mTagBFP2-6mer was a gift from Joanna Wysocka (Addgene plasmid # 253417 ; http://n2t.net/addgene:253417 ; RRID:Addgene_253417)

  • For your References section:

    Promoter strength and position govern promoter competition through transcript-dependent insulation. Koska M, Nagano M, Swigut T, Boettiger AN, Hansen AS, Wysocka J. Nat Genet. 2026 Jul 20. doi: 10.1038/s41588-026-02691-y. 10.1038/s41588-026-02691-y PubMed 42477113
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