pHLP785
(Plasmid
#253572)
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PurposeIntegration of OsTIR1(F74A)-3Myc expressed from C. albicans TDH3 promoter with recyclable SAT1 (nourseothricin) selection marker in C. albicans for use in AID2 system.
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253572 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepBluescript II SK+
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Vector typeYeast Expression
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameOsTIR1(F74A)-3Myc
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SpeciesOryza sativa
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MutationF74A variant of Oryza sativa TIR1 gene
Cloning Information
- Cloning method Ligation Independent Cloning
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pHLP785 was a gift from Mark Hall (Addgene plasmid # 253572 ; http://n2t.net/addgene:253572 ; RRID:Addgene_253572)