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pOS1T-Mb(IPYE)PylRSτMH
(Plasmid #253665)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253665 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pOS1T
  • Backbone manufacturer
    in-house design and optimization
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 10 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Mb(IPYE)PylRS-τMH
  • Alt name
    τMH-RS

Gene/Insert 2

  • Gene/Insert name
    MsPylT_CUA
  • Alt name
    Ms-Pyl-tRNA_CUA

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.1101/2025.11.04.686677 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pOS1T-Mb(IPYE)PylRSτMH was a gift from Alexandria Deliz Liang (Addgene plasmid # 253665 ; http://n2t.net/addgene:253665 ; RRID:Addgene_253665)

  • For your References section:

    Genetic Incorporation of Diverse Noncanonical Amino Acids for Histidine Substitution. Natter Perdiguero A, Fischer S, Lischke AR, Manser BP, Deliz Liang A. J Am Chem Soc. 2026 Apr 8;148(13):13619-13632. doi: 10.1021/jacs.5c19599. Epub 2026 Mar 26. 10.1021/jacs.5c19599 PubMed 41885601
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