Genetic Incorporation of Diverse Noncanonical Amino Acids for Histidine Substitution.
Natter Perdiguero A, Fischer S, Lischke AR, Manser BP, Deliz Liang A
J Am Chem Soc. 2026 Apr 8;148(13):13619-13632. doi: 10.1021/jacs.5c19599. Epub 2026 Mar 26.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253651 | pOS1T-chPheRS-4PyA-3C11-chPheT_CUA | Production of engineered aaRS/tRNA pair for incorporation of 4PyA |
| 253654 | pOS1T-MmPylRS123Trz-4A | Production of engineered aaRS/tRNA pair for incorporation of 123Trz-4A |
| 253655 | pOS1T-chPylRS5NO2H | Production of engineered aaRS/tRNA pair for incorporation of 5NO2H |
| 253656 | pOS1T-chPylRS3PzA | Production of engineered aaRS/tRNA pair for incorporation of 3PzA |
| 253657 | pOS1T-chPylR2E5MH | Production of engineered aaRS/tRNA pair for incorporation of 2E5MH |
| 253658 | pOS1T-chPylRS2PyA | Production of engineered aaRS/tRNA pair for incorporation of 2PyA |
| 253659 | pOS1T-MmPylRSτBnH | Production of engineered aaRS/tRNA pair for incorporation of τBnH (as well as 1Bn123Trz-4A) |
| 253663 | pOS1T-chPylRS4OxzA | Production of engineered aaRS/tRNA pair for incorporation of 4OxzA |
| 253665 | pOS1T-Mb(IPYE)PylRSτMH | Production of engineered aaRS/tRNA pair for incorporation of τMH. Less effective than the two-plasmid system |
| 253666 | pSLdT7-Mb(IPYE)PylRSτMH | Production of engineered aaRS for incorporation of τMH (part of two plasmid system) |
| 253667 | pDPS2-MstRNAPyl_CUA | Production of tRNA for incorporation of τMH (part of two plasmid system) |
| 253668 | pOS1T-MbPylRS4ThzA | Production of engineered aaRS/tRNA pair for incorporation of 4ThzA |
| 253669 | pOS1T-MmPylRS(7_1) | Production of engineered aaRS/tRNA pair for incorporation of 3ThA |
| 253670 | pOS1T-MmPylRS(8_2) | Production of engineered aaRS/tRNA pair for incorporation of 3PyA |
| 253671 | pos1t-G1PylRSMIFAF-G1PylTCUA | Production of engineered aaRS/tRNA pair for incorporation of πMH |
| 253672 | pOS1Tcc-G1PylRSMIFAF-MatRNAPyl(8)UCA | Production of engineered aaRS/tRNA pair for incorporation of πMH (opal suppression) |