Skip to main content
Addgene
Open Menu

PB-TetO-PEmax-2A-MLH1dn-2A-BFP
(Plasmid #253728)

Print

Ordering

This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253728 Standard format: Plasmid sent in bacteria as agar stab 1 $94
Add to Cart

Backbone

  • Vector backbone
    PB_TetO_Hygro_GW
  • Selectable markers
    Hygromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    PEmax
  • Tags / Fusion Proteins
    • BFP
    • MLH1dn

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The plasmid was cloned using three inserts from the following plasmids: PEmax: https://www.addgene.org/174820/; MLH1dn: https://www.addgene.org/174827/sequences/; BFP: https://www.addgene.org/46911/. The original plasmids were purchased from Addgene. There is an Evrogen limited use label licence for the fluorescent protein:

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    PB-TetO-PEmax-2A-MLH1dn-2A-BFP was a gift from Alex Radzisheuskaya (Addgene plasmid # 253728 ; http://n2t.net/addgene:253728 ; RRID:Addgene_253728)

  • For your References section:

    Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing. Price D, Zemlyanskiy G, Trakarnphornsombat W, Forne I, Volkova N, Dubusse L, Cooper AJ, Imhof A, Radzisheuskaya A. Nat Genet. 2026 Jul 8. doi: 10.1038/s41588-026-02675-y. 10.1038/s41588-026-02675-y PubMed 42420522
Related items: