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pL-CRISPR-SFFV-Puro-P2A-EGFP_peg
(Plasmid #253729)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253729 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pL-CRISPR-SFFV-Puro-P2A-EGFP
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was cloned based on https://www.addgene.org/175037/ by introducing a 3-nucleotide mutation. The original plasmid was purchased from Addgene.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pL-CRISPR-SFFV-Puro-P2A-EGFP_peg was a gift from Alex Radzisheuskaya (Addgene plasmid # 253729 ; http://n2t.net/addgene:253729 ; RRID:Addgene_253729)

  • For your References section:

    Identifying critical lysines in mammalian histone H3 with high-throughput CRISPR prime editing. Price D, Zemlyanskiy G, Trakarnphornsombat W, Forne I, Volkova N, Dubusse L, Cooper AJ, Imhof A, Radzisheuskaya A. Nat Genet. 2026 Jul 8. doi: 10.1038/s41588-026-02675-y. 10.1038/s41588-026-02675-y PubMed 42420522
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