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Protrudin-WT
(Plasmid #253736)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253736 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pLXIN
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    WT
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1900
  • Mutation
    WT
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)
  • Promoter CMV

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Protrudin-WT was a gift from Evan Reid (Addgene plasmid # 253736 ; http://n2t.net/addgene:253736 ; RRID:Addgene_253736)

  • For your References section:

    Protrudin acts at ER-endosome contacts to promote KIF5-mediated endosomal tubule fission. Kleniuk J, Nadadhur AG, Rodger C, Wolfenden E, Hall IA, Cheers SR, Zlamalova E, Reid E. Neurobiol Dis. 2026 Jan;218:107231. doi: 10.1016/j.nbd.2025.107231. Epub 2025 Dec 20. 10.1016/j.nbd.2025.107231 PubMed 41429245
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