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pJF34
(Plasmid #253752)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253752 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pYTK-DN4
  • Backbone manufacturer
    Andreas Milias-Argeitis, Addgene Plasmid #180285 as described by Novarina et al. (2022).
  • Vector type
    Yeast Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    GFP_AID*
  • Alt name
    pTEF1-GFP_AID*-tCYC1
  • Insert Size (bp)
    4569
  • Promoter TEF1
  • Tag / Fusion Protein
    • mAID*-tag (C terminal on insert)

Cloning Information for Gene/Insert 1

Gene/Insert 2

  • Gene/Insert name
    OsTIR1 (non codon optimized)
  • Alt name
    pADH1-OsTIR1(F74G)-tPGK1
  • Mutation
    F74G
  • Promoter ADH1

Cloning Information for Gene/Insert 2

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    GFP-AID* sequence was obtained from plasmid pG2J (Addgene plasmid #102882) described in Papagiannakis et al. (2017) Molecular Cell, 10.1016/j.molcel.2016.11.018.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The golden gate (Multi-gene) plasmid assembly was performed according to the MoClo-Cloning standards described in: Lee, M. E., DeLoache, W. C., Cervantes, B., & Dueber, J. E. (2015). A highly characterized yeast toolkit for modular, multipart assembly. ACS synthetic biology, 4(9), 975-986.

Parts from the MoClo Yeast Toolkit (Addgene kit #1000000061) were used for MoClo assembly.

The OsTIR1(F74G) variant was originally described in Yesbolatova et al., 2020 (https://doi.org/10.1038/s41467-020-19532-z)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pJF34 was a gift from Matthias Heinemann (Addgene plasmid # 253752 ; http://n2t.net/addgene:253752 ; RRID:Addgene_253752)
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