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pcDNA3.4
(Plasmid #253816)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253816 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pcDNA3.4
  • Backbone manufacturer
    GenScript
  • Backbone size (bp) 6011
  • Vector type
    Mammalian Expression
  • Promoter CMV
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gene Synthesis

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Synthesized through GeneScript.

Please visit https://doi.org/10.1101/2025.10.15.682650 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.4 was a gift from Bess Frost (Addgene plasmid # 253816 ; http://n2t.net/addgene:253816 ; RRID:Addgene_253816)

  • For your References section:

    Elevation of the mechanically-sensitive e protein emerin links nuclear mechanotransduction to tau-induced cytoskeletal remodeling in neurons. Sohn C, Pardo S, Molleur D, Paduri SR, Lambert M, Uttke Z, Sohn EJ, Thomas MG, Weintraub ST, Frost B. Nucleus. 2026 Dec;17(1):2697135. doi: 10.1080/19491034.2026.2697135. Epub 2026 Jul 7. 10.1080/19491034.2026.2697135 PubMed 42415348
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