pPD793 DsRE2 driven by TETON3G
(Plasmid
#253880)
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PurposeIntegration Vector for DsRE2 reporter for the TRE3GV promoter (TUPV1), Tet-ON 3G with the CAG promtor (TUPV2), and EBFP2-P2A-BlastR with the CAG promoter (TUPV3)
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253880 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepPD630 Integration Vector
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Vector typeMammalian Expression, Synthetic Biology
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Selectable markersBlasticidin
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDsRE2, TetON3G, EBFP2-P2A-BlastR
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SpeciesSynthetic
- Promoter TRE3GV, CAG, CAG
Cloning Information
- Cloning method Golden Gate
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Leonard Lab plasmid reference number: L3124
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPD793 DsRE2 driven by TETON3G was a gift from Joshua Leonard (Addgene plasmid # 253880 ; http://n2t.net/addgene:253880 ; RRID:Addgene_253880) -
For your References section:
Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors. Dreyer KS, Donahue PS, Boucher JD, Chambers KM, Ornelas MY, Edelstein HI, Leibowitz BD, Zhu KJ, Dray KE, Muldoon JJ, Leonard JN. ACS Synth Biol. 2025 May 16;14(5):1464-1481. doi: 10.1021/acssynbio.4c00714. Epub 2025 May 2. 10.1021/acssynbio.4c00714 PubMed 40315428