Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors.
Dreyer KS, Donahue PS, Boucher JD, Chambers KM, Ornelas MY, Edelstein HI, Leibowitz BD, Zhu KJ, Dray KE, Muldoon JJ, Leonard JN
ACS Synth Biol. 2025 May 16;14(5):1464-1481. doi: 10.1021/acssynbio.4c00714. Epub 2025 May 2.
(Link opens in a new window)
PubMed
(Link opens in a new window)
Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253871 | pPD783 U6 sgRNA Rosa26 Cas9-T2A-BFP-P2A-Ad4E4orf6 | NHEJ Inhibitor 2 with Cas9 and Rosa26 sgRNA |
| 253874 | pPD211 HBS CMV_min EYFP | EYFP reporter for the HBS(CMV_min) promoter |
| 253875 | pPD214 HBS YB_TATA EYFP | EYFP reporter for the HBS(YB_TATA) promoter |
| 253876 | pPD217 HBS SV40_min EYFP | EYFP reporter for the HBS(SV40_min) promoter |
| 253880 | pPD793 DsRE2 driven by TETON3G | Integration Vector for DsRE2 reporter for the TRE3GV promoter (TUPV1), Tet-ON 3G with the CAG promtor (TUPV2), and EBFP2-P2A-BlastR with the CAG promoter (TUPV3) |
| 253881 | pPD766 TRE3GV DsRE2 in TUPV1 | Components for Integration Vectors: mMoClo TUPV, with TRE3GV promoter and DsRe2 in the MCS |
| 253882 | pPD769 TRE3GV HIF1a in TUPV2 | Components for Integration Vectors: mMoClo TUPV, with TRE3GV promoter and HIF1a* in the MCS |
| 253883 | pPD770 Tet-On 3G in TUPV3 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and TetOn3G |
| 253884 | pPD847 Tet On 3G in TU2 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and TetOn3G |
| 253885 | pPD774 HBS CMV_min DsRE2 in TUPV1 | Components for Integration Vectors: mMoClo TUPV, with HBS(CMV_min) promoter and DsRE2 |
| 253886 | pPD775 HBS YB_TATA DsRE2 in TUPV1 | Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and DsRE2 |
| 253887 | pPD852 EBFP2 P2A Blast in TUPV2 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and EBFP2-2A-BlastR |
| 253888 | pPD853 EBFP2 P2A Blast in TUPV3 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and EBFP2-2A-BlastR |
| 253889 | pPD854 EBFP2 P2A Blast in TUPV4 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and EBFP2-2A-BlastR |
| 253890 | pPD1401 Only mTagBFP2 | Integration Vector for AkaLuc-P2A-mTagBFP-P2A-BlastR reporter with CAG promoter |
| 253891 | pPD1402 Dox-Inducible mNeonGreen | Integration Vector for mNeonGreen with the TRE3GV promoter (TUPV1), TetOn3G with the CAG promoter (TUPV2), and BlastR with the CAG promoter (TUPV3) |
| 253903 | pPD1043 TRE3GV mTagBFP2 | Components for Integration Vectors: mMoClo TUPV, with TRE3GV promoter and mTagBFP2 in the MCS |
| 253904 | pPD1031 EF1a miRFP670 2A Blast | Components for Integration Vectors: mMoClo TUPV, with EF1alpha promoter and miRFP720-P2A-BlastR in the MCS |
| 253905 | pPD1354 TRE3GV LumiScarlet in TU1 | Components for Integration Vectors: mMoClo TUPV, with TRE3GV promoter and LumiScarlet in the MCS |
| 253906 | pPD1351 HBS(CMV) LumiScarlet | Components for Integration Vectors: mMoClo TUPV, with HBS(CMV_min) promoter and LumiScarlet in the MCS |
| 253907 | pPD1352 HBS(SV40) LumiScarlet | Components for Integration Vectors: mMoClo TUPV, with HBS(SV40_min) promoter and LumiScarlet in the MCS |
| 253908 | pPD1350 HBS(YBTATA) LumiScarlet | Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and LumiScarlet in the MCS |
| 253909 | pPD1357 HBS(YBTATA) LumiScarlet in TU2 | Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and LumiScarlet in the MCS |
| 253910 | pPD1358 HBS(YBTATA) LumiScarlet in TU3 | Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and LumiScarlet in the MCS |
| 253911 | pPD1359 HBS(YBTATA) LumiScarlet in TU4 | Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and LumiScarlet in the MCS |
| 253912 | pPD1271 HBS(Ben) mHIF1a in TU2 | Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and mHIF1_ in the MCS |
| 253913 | pPD1272 HBS(Ben) mHIF2a in TU2 | Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and mHIF2_ in the MCS |
| 253914 | pPD1284 HBS(Ben) mHIF1beta in TU3 | Components for Integration Vectors: mMoClo TUPV, with HBS(YB_TATA) promoter and mHIF1_ in the MCS |
| 253916 | pPD1286 CAG TetON 3G in TU4 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and TetON3G in the MCS |
| 253917 | pPD1361 CAG Blast R in TU3 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and BlastR in the MCS |
| 253918 | pPD1291 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU1 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS |
| 253919 | pPD1292 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU2 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS |
| 253920 | pPD1293 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU3 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS |
| 253921 | pPD1294 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU4 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS |
| 253922 | pPD1295 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU5 | Components for Integration Vectors: mMoClo TUPV, with CAG promoter and mTagBFP2-P2A-AkaLuc-2A-BlastR in the MCS |