pPD766 TRE3GV DsRE2 in TUPV1
(Plasmid
#253881)
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PurposeComponents for Integration Vectors: mMoClo TUPV, with TRE3GV promoter and DsRe2 in the MCS
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253881 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
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Vector backbonepPD561 CAG MCS TUPV1
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Vector typeMammalian Expression, Synthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDsRE2
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SpeciesSynthetic
- Promoter TRE3GV
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site Unknown (unknown if destroyed)
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Leonard Lab plasmid reference number: L3098
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pPD766 TRE3GV DsRE2 in TUPV1 was a gift from Joshua Leonard (Addgene plasmid # 253881 ; http://n2t.net/addgene:253881 ; RRID:Addgene_253881) -
For your References section:
Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors. Dreyer KS, Donahue PS, Boucher JD, Chambers KM, Ornelas MY, Edelstein HI, Leibowitz BD, Zhu KJ, Dray KE, Muldoon JJ, Leonard JN. ACS Synth Biol. 2025 May 16;14(5):1464-1481. doi: 10.1021/acssynbio.4c00714. Epub 2025 May 2. 10.1021/acssynbio.4c00714 PubMed 40315428