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pPD1432 HBS HIF2a feedback
(Plasmid #253900)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253900 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pPD1178 Integration Vector (PuroR-P2A-miRFP720)
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LumiScarlet, mHIF2_, AkaLuc, mTagBFP2 and BlastR
  • Species
    Synthetic
  • Promoter HBS(YB_TATA), HBS(YB_TATA), CAG

Cloning Information

  • Cloning method Golden Gate

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Leonard Lab plasmid reference number: L3482

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPD1432 HBS HIF2a feedback was a gift from Joshua Leonard (Addgene plasmid # 253900 ; http://n2t.net/addgene:253900 ; RRID:Addgene_253900)

  • For your References section:

    Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors. Dreyer KS, Donahue PS, Boucher JD, Chambers KM, Ornelas MY, Edelstein HI, Leibowitz BD, Zhu KJ, Dray KE, Muldoon JJ, Leonard JN. ACS Synth Biol. 2025 May 16;14(5):1464-1481. doi: 10.1021/acssynbio.4c00714. Epub 2025 May 2. 10.1021/acssynbio.4c00714 PubMed 40315428
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