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pPD1295 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU5
(Plasmid #253922)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253922 Standard format: Plasmid sent in bacteria as agar stab 1 $89
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Backbone

  • Vector backbone
    pPD565 CAG MCS TUPV5
  • Vector type
    Mammalian Expression, Synthetic Biology
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mTagBFP2-P2A-AkaLuc-2A-BlastR
  • Species
    Synthetic
  • Promoter CAG

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site NotI (not destroyed)

Resource Information

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Leonard Lab plasmid reference number: L3444

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPD1295 CAG AkaLuc-P2A-mTagBFP2-P2A-BlastR in TU5 was a gift from Joshua Leonard (Addgene plasmid # 253922 ; http://n2t.net/addgene:253922 ; RRID:Addgene_253922)

  • For your References section:

    Engineered Feedback Employing Natural Hypoxia-Responsive Factors Enhances Synthetic Hypoxia Biosensors. Dreyer KS, Donahue PS, Boucher JD, Chambers KM, Ornelas MY, Edelstein HI, Leibowitz BD, Zhu KJ, Dray KE, Muldoon JJ, Leonard JN. ACS Synth Biol. 2025 May 16;14(5):1464-1481. doi: 10.1021/acssynbio.4c00714. Epub 2025 May 2. 10.1021/acssynbio.4c00714 PubMed 40315428
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