pCumate-sfGFP
(Plasmid
#253929)
-
PurposeoripBBR1, mob+, plasmid derived from pMLBAD containing the cumate-inducible system PCym/CymR controlling sfGFP, TmpR.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253929 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $89 | |
Backbone
-
Vector backbonepMLBAD (Addgene #32055)
-
Backbone manufacturerMiguel Valvano
-
Vector typeBacterial Expression
Growth in Bacteria
-
Bacterial Resistance(s)Trimethoprim, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberLow Copy
Gene/Insert
-
Gene/Insert nameCymR inducible systems
Resource Information
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.11.26.690688 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pCumate-sfGFP was a gift from Nichollas Scott (Addgene plasmid # 253929 ; http://n2t.net/addgene:253929 ; RRID:Addgene_253929)