Nichollas Scott Lab: Generation of an optimised cumate toolkit for tuneable protein expression during in vitro and in vivo studies of Burkholderia cenocepacia
Tahir H, Karlic KI, Mwiti G, Scott NE
bioRxiv 2025.11.26.690688
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 253929 | pCumate-sfGFP | oripBBR1, mob+, plasmid derived from pMLBAD containing the cumate-inducible system PCym/CymR controlling sfGFP, TmpR. |
| 253930 | pCumateGV-sfGFP | oripBBR1, mob+, plasmid derived from pMLBAD containing the cumate-inducible system PCymRC and the codon optimised CymRGV controlling sfGFP, TmpR. |
| 253931 | pCumateGV-MCS | oripBBR1, mob+, plasmid derived from pMLBAD containing the cumate-inducible system PCymRC and the codon optimised CymRGV upstream of an MCS, TmpR. |
| 253932 | pCTX-CymRGV-MCS | CTX based integration vector containing PCymRC and the codon optimised CymRGV. Derived from pAH-CTX-Rha replacing the rhamnose induction circuit with PCymRC/CymRGV upstream of a MCS, tetR. |
| 253933 | pCTX-CymRGV-sfGFP | CTX based integration vector containing PCymRC and the codon optimised CymRGV. Derived from pAH-CTX-Rha replacing the rhamnose induction circuit with PCymRC/CymRGV controlling sfGFP, tetR. |