12xHis-SUMO-SMARCA2(1373-1509) Brd only
(Plasmid
#253938)
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Purposefor Bacterial Protein Overexpression
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 253938 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepRSF-DUET1
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Kanamycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
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Alt nameSWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 2
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SpeciesH. sapiens (human)
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Entrez GeneSMARCA2 (a.k.a. BAF190, BIS, BRM, NCBRS, SAMRCA2, SNF2, SNF2L2, SNF2LA, SWI2, Sth1p, hBRM, hSNF2a)
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Tag
/ Fusion Protein
- 12x His SUMO
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer T7 forward
- (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.1101/2025.08.04.668513 for the bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
12xHis-SUMO-SMARCA2(1373-1509) Brd only was a gift from Alessio Ciulli (Addgene plasmid # 253938 ; http://n2t.net/addgene:253938 ; RRID:Addgene_253938) -
For your References section:
Dual E3 ligase recruitment by monovalent degraders for tunable SMARCA 2/4 degradation. Spiteri VA, Segal D , Correa-Sáez A, Iso K, Casement R, Muñoz i Ordoño M, Nakasone MA, Sathe G, Schätz C, Peters HE, Doward M, Kainacher L, Cowan AD, Ciulli A, Winter GE. Nat Chem Biol (2026). doi: 10.1038/s41589-026-02224-y 10.1038/s41589-026-02224-y