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pSBtet-GP-α7-NACHO_EM
(Plasmid #253979)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253979 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pSBtet-GP
  • Backbone manufacturer
    Eric Kowarz (Addgene plasmid # 60495)
  • Backbone size w/o insert (bp) 6300
  • Total vector size (bp) 8699
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Human alpha7 nicotinic acetylcholine receptor
  • Alt name
    CHRNA7
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2373
  • Mutation
    Deletion in M3M4 loop with M3M4 loop insertion of bRIL. C-terminus of a7 has twin strep, followed by T2A sequence, then nAChO gene
  • Entrez Gene
    CHRNA7 (a.k.a. CHRNA7-2, NACHRA7, nAChR7)
  • Tag / Fusion Protein
    • Twin strep tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SfiI (not destroyed)
  • 3′ cloning site SfiI (not destroyed)
  • 5′ sequencing primer gccaattccaactcttttg
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    The vector came from Addgene (Kowarz lab).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSBtet-GP-α7-NACHO_EM was a gift from Ryan Hibbs (Addgene plasmid # 253979 ; http://n2t.net/addgene:253979 ; RRID:Addgene_253979)

  • For your References section:

    Structural mechanisms of alpha7 nicotinic receptor allosteric modulation and activation. Burke SM, Avstrikova M, Noviello CM, Mukhtasimova N, Changeux JP, Thakur GA, Sine SM, Cecchini M, Hibbs RE. Cell. 2024 Feb 29;187(5):1160-1176.e21. doi: 10.1016/j.cell.2024.01.032. Epub 2024 Feb 20. 10.1016/j.cell.2024.01.032 PubMed 38382524
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