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pET-51b(+)-twinstrep-RBBP6 NTD
(Plasmid #253991)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 253991 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pET-51b(+)
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    RBBP6
  • Species
    H. sapiens (human)
  • Mutation
    includes NTD only (aa 1-350)
  • Entrez Gene
    RBBP6 (a.k.a. MY038, P2P-R, PACT, RBQ-1, SNAMA)
  • Promoter T7
  • Tags / Fusion Proteins
    • TwinStrep (N terminal on insert)
    • 10xHis (C terminal on backbone)

Cloning Information

  • Cloning method Ligation Independent Cloning

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET-51b(+)-twinstrep-RBBP6 NTD was a gift from Yongsheng Shi (Addgene plasmid # 253991 ; http://n2t.net/addgene:253991 ; RRID:Addgene_253991)

  • For your References section:

    RBBP6 anchors pre-mRNA 3' end processing to nuclear speckles for efficient gene expression. Yoon Y, Bournique E, Soles LV, Yin H, Chu HF, Yin C, Zhuang Y, Liu X, Liu L, Jeong J, Yu C, Valdez M, Tian L, Huang L, Shi X, Seelig G, Ding F, Tong L, Buisson R, Shi Y. Mol Cell. 2025 Feb 6;85(3):555-570.e8. doi: 10.1016/j.molcel.2024.12.016. Epub 2025 Jan 10. 10.1016/j.molcel.2024.12.016 PubMed 39798570
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