NK812
(Plasmid #253995)

• Purpose: Encodes the 3’ end of S. rosetta PTSG_07215 CDS (without STOP codon) fused to a linker and followed by mStayGold CDS and the 3’ UTR region of S. rosetta PTSG_07215.
• Depositing Lab: Nicole King
• Publication: Carver et al bioRxiv [Preprint]. 2026 Apr 7:2026.04.05.716596. doi: 10.64898/2026.04.05.716596.

Backbone

• Vector backbone: pMS18
• Backbone size w/o insert (bp): 4570
• Total vector size (bp): 5558
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: PTSG_07215_3’CDS-recovery-sequence-linker-mStayGold-PTSG_07215-3’UTR
• Alt name: PTSG_07215-mStayGold
• Species: Salpingoeca rosetta
• Insert Size (bp): 988
• Mutation: Synonymous codon mutations made to PTSG_07215 3’ CDS region (described in publication and marked on the annotated .gb supplemental document)
• GenBank ID: XP_004992637.1
• Tag / Fusion Protein:
  • mStayGold (C terminal on insert)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: ATGACCATGATTACGCCAAGCTTAAGAAGAAAAGCGTAAGGCTGC
• 3′ sequencing primer: AAGACTGCAGTCGTTACGTTTCCATATGCGATGTATGTGT

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry