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mSwAP-In_MC2v2(Rosa26)_Acceptor (pKBA204)
(Plasmid #254036)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254036 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    Unspecified
  • Backbone size w/o insert (bp) 18500
  • Vector type
    Mammalian Expression, Synthetic Biology ; Mouse Targeting
  • Selectable markers
    Neomycin (select with G418) ; HSV-dTK (counterselect with ganciclovir)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    EPI300
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    mNeonGree-P2A-FCU1-T2A-NeoR
  • Alt name
    mNeonGreen: bright monomeric yellow-green fluorescent protein derived from LanYFP
  • Alt name
    FCU1: fusion of yeast FCY1 and FUR1 genes; NeoR: aminoglycoside phosphotransferase from Tn5
  • Species
    Multiple species
  • Insert Size (bp)
    4500
  • Promoter human EF1a

Cloning Information

  • Cloning method Unknown

Terms and Licenses

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

This is a derivative of mSwAP-In MC2v2 (pKBA135) that has Rosa26-targeting homology arms and a multiple cloning site (MCS) for assembling payloads.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mSwAP-In_MC2v2(Rosa26)_Acceptor (pKBA204) was a gift from Jef Boeke (Addgene plasmid # 254036 ; http://n2t.net/addgene:254036 ; RRID:Addgene_254036)

  • For your References section:

    Improved vector toolkit for genome writing in mammalian cells. Barriball K, Berrios B, Coelho C, Pinglay S, Zhao Y, Chalhoub N, Tsou T, Atwater JT, Boeke JD, Zhang W, Brosh R. bioRxiv [Preprint]. 2026 Mar 17:2026.03.15.711894. doi: 10.64898/2026.03.15.711894. 10.64898/2026.03.15.711894 PubMed 41890105
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