Improved vector toolkit for genome writing in mammalian cells.
Barriball K, Berrios B, Coelho C, Pinglay S, Zhao Y, Chalhoub N, Tsou T, Atwater JT, Boeke JD, Zhang W, Brosh R
bioRxiv [Preprint]. 2026 Mar 17:2026.03.15.711894. doi: 10.64898/2026.03.15.711894.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254034 | pLP-TK (pCTC174) | mSwAP-In/Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-dTK-P2A-HaloTag expression cassette flanked by LoxM/LoxP sites and BsaI homology arms cloning sites. FCU1-counterselectable backbone. |
| 254035 | mSwAP-In MC2v2 (pKBA135) | mSwAP-In payload acceptor vector for delivery of large DNA constructs to mammalian cells. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction. |
| 254037 | pSpCas9 UGT1 (pWZ594) | Express SpCas9 and the UGT1 gRNA |
| 254039 | pSpCas9 LP400~3p (pKBA314) | Express SpCas9 and the LP400~3p gRNA |
| 254040 | pSpCas9 UNS1 (pKBA244) | Express SpCas9 and the UNS1 gRNA |
| 254041 | pSpCas9 UNS2 (pKBA235) | Express SpCas9 and the UNS2 gRNA |
| 254042 | pSpCas9 UGT1+LP400~3p (pNCH0155) | Express SpCas9 and two gRNAs: UGT1 and LP400~3p |