pLP-TK (pCTC174)
(Plasmid #254034)

• Purpose: mSwAP-In/Big-IN Landing Pad plasmid encoding pEF1a-driven PuroR-dTK-P2A-HaloTag expression cassette flanked by LoxM/LoxP sites and BsaI homology arms cloning sites. FCU1-counterselectable backbone.
• Depositing Lab: Jef Boeke
• Publication: Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.15.711894. doi: 10.64898/2026.03.15.711894.

Backbone

• Vector backbone: Unspecified
• Backbone size w/o insert (bp): 5700
• Vector type: Mammalian Expression, Synthetic Biology ; Mouse Targeting, Recombinase-mediated cassette exchange (RMCE) Landing Pad
• Selectable markers: Puromycin ; FCU1 (counterselect with 5-FC)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: PuroR-dTK-P2A-HaloTag
• Alt name: PuroR: Streptomyces alboniger Puromycin N-acetyltransferase
• Alt name: dTK: truncated herpes simplex virus thymidine kinase
• Species: Multiple species
• Insert Size (bp): 4400
• Promoter: human EF1a

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is an "empty" LP plasmid. Homology arms for genome targeting are added using BsaI-mediated Golden Gate cloning. Backbone contains bacterial selection marker AmpR and yeast selection marker HIS5.