mSwAP-In MC2v2 (pKBA135)
(Plasmid #254035)

• Purpose: mSwAP-In payload acceptor vector for delivery of large DNA constructs to mammalian cells. Encodes LEU2 yeast selectable marker and components for bacterial copy number induction.
• Depositing Lab: Jef Boeke
• Publication: Barriball et al bioRxiv [Preprint]. 2026 Mar 17:2026.03.15.711894. doi: 10.64898/2026.03.15.711894.

Backbone

• Vector backbone: Unspecified
• Backbone size w/o insert (bp): 15600
• Vector type: Mammalian Expression, Synthetic Biology ; Mouse Targeting
• Selectable markers: Neomycin (select with G418) ; HSV-dTK (counterselect with ganciclovir)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): EPI300
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: mNeonGree-P2A-FCU1-T2A-NeoR
• Alt name: mNeonGreen: bright monomeric yellow-green fluorescent protein derived from LanYFP
• Alt name: FCU1: fusion of yeast FCY1 and FUR1 genes; NeoR: aminoglycoside phosphotransferase from Tn5
• Species: Multiple species
• Insert Size (bp): 4500
• Promoter: human EF1a

Cloning Information

• Cloning method: Unknown

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • mNeonGreen Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This is an empty Big DNA assembly and delivery vector. Homology arms for genome targeting are added using NotI-mediated Gibson assembly. Big DNA Payloads are cloned using yeast assembly or recombineering. This plasmid can be copy-number induced using Arabinose. Backbone contains bacterial selection marker KanR and yeast selection marker LEU2.