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pSpCas9 UNS2 (pKBA235)
(Plasmid #254041)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254041 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pX330
  • Backbone size w/o insert (bp) 8500
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA_UNS2
  • gRNA/shRNA sequence
    CTTCGCTGGGAGTTCGTAGA
  • Species
    Synthetic
  • Insert Size (bp)
    20
  • Promoter U6

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid express a gRNA driven by a human U6 promoter and a CAG promoter-driven SpCas9-NLS.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSpCas9 UNS2 (pKBA235) was a gift from Jef Boeke (Addgene plasmid # 254041 ; http://n2t.net/addgene:254041 ; RRID:Addgene_254041)

  • For your References section:

    Improved vector toolkit for genome writing in mammalian cells. Barriball K, Berrios B, Coelho C, Pinglay S, Zhao Y, Chalhoub N, Tsou T, Atwater JT, Boeke JD, Zhang W, Brosh R. bioRxiv [Preprint]. 2026 Mar 17:2026.03.15.711894. doi: 10.64898/2026.03.15.711894. 10.64898/2026.03.15.711894 PubMed 41890105
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