TCSn-HaloTag-TM1-mCherry
(Plasmid #254126)

• Purpose: Cytokinin-sensitive transcriptional reporter used for magnetic sorting of plant protoplasts following gene perturbation.
• Depositing Lab: Elizabeth Sattely
• Publication: Lowensohn et al bioRxiv 2025.08.06.668818

Backbone

• Vector backbone: pBIN20
• Vector type: Plant Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: HaloTag-TEV-transmembrane domain-mCherry
• Alt name: MAPS reporter
• Species: Synthetic
• Insert Size (bp): 891
• Promoter: CaMV 35S

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Transmembrane domain was received as a gift from the X. Gao Lab at Stanford University (Vlahos et al., 2025 https://doi.org/10.1101/2023.10.04.560774).

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please see the following preprints as well as their final/published form:

Lowensohn, T. N.; Cody, W. B.; Tsai, C.; Vlahos, A. E.; Call, C. C.; Gao, X. J.; Sattely, E. S. An in Planta Single-Cell Screen to Accelerate Functional Genetics. bioRxiv February 19, 2026, p 2025.08.06.668818. https://doi.org/10.1101/2025.08.06.668818.

Vlahos, A. E.; Call, C. C.; Eckman, N.; Kadaba, S. E.; Guo, S.; Thurm, A. R.; Kang, J.; Appel, E. A.; Bintu, L.; Gao, X. J. A Complete Suite for Compact Programming of Protein Secretion. bioRxiv May 27, 2025, p 2023.10.04.560774. https://doi.org/10.1101/2023.10.04.560774.