pET29b(+)_SUMO(1-98)_sTEV_His6
(Plasmid #254139)

• Purpose: Plasmid for expressing Control protein construct of the AH-SUMO platfrom, without any N-terminal inducible amphipathic helix.
• Depositing Lab: Peter Chung
• Publication: Margaritakis et al Commun Chem. 2026 Apr 2;9(1):165. doi: 10.1038/s42004-026-01994-9.

Backbone

• Vector backbone: pET29b(+)
• Backbone size w/o insert (bp): 5228
• Total vector size (bp): 5582
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: SUMO family protein SMT3
• Species: S. cerevisiae (budding yeast)
• Insert Size (bp): 354
• Mutation: Contains MGSGS sequence on N-terminal of SMT3_yeast SUMO(1-98) gene. Gene is followed by a Cysteine, a TEV site and a hexahistidine tag.
• Entrez Gene: SMT3 (a.k.a. YDR510W)

Cloning Information

• Cloning method: Gibson Cloning
• 5′ sequencing primer: Cloned into cloning vector pET29b(+)_NdeI-NcoI_SUMO(21-98)_CV

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains a SUMO protein construct to serve as a control protein for binding experiments using the AH-SUMO platform, to verify non-interaction between the SUMO domain and liposomes of choice. It contains the SUMO1-98 region of the SMT3_yeast gene, followed by a Cysteine, a TEV site and a hexa-histidine site. On the N-terminal of the SUMO domain, it contains a region encoding for MGSGS, which is there to mimic the structure of a AH-SUMO platform that contains an N-terminal peptide of interest , serving as a better control.

Please visit https://doi.org/10.1101/2025.05.12.653516 for the bioRxiv preprint.