Recombinant protein platform for high-throughput investigation of peptide-liposome interactions via fluorescence anisotropy depolarization.

Margaritakis A, Qian M, Johnson DH, Zeno WF, Ulmer TS, Chung PJ
Commun Chem. 2026 Apr 2;9(1):165. doi: 10.1038/s42004-026-01994-9. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
254139	pET29b(+)_SUMO(1-98)_sTEV_His6	Plasmid for expressing Control protein construct of the AH-SUMO platfrom, without any N-terminal inducible amphipathic helix.
254142	pET29b(+)_NdeI-NcoI_SUMO(21-98)_CV	Vector for cloning in inducible amphipathic helix of choice to the N-terminal of the AH-SUMO platform. Clone using HiFi assembly between NdeI-NcoI and add "GSGSMSDSEVNQEAKPEVKPEVKP" to end of peptide.