pET28a HaloTag-TEVc-empty
(Plasmid #254152)

• Purpose: (Empty Backbone) Backbone for bacterial expression with N-terminal HaloTag and a TEV-protease cleavage site
• Depositing Lab: Rahul Samant
• Depositing Organization: Babraham Institute
  Located in United Kingdom of Great Britain and Northern Ireland
• Publication: Johnston et al bioRxiv 2026.02.13.703496

Backbone

• Vector backbone: pET28a
• Backbone manufacturer: Novagen; MRC-PPU Reagents & Services, UK; Daniel Daley
• Backbone size (bp): 6197
• Modifications to backbone: Optimised T7 promoter (from Addgene # 154464); HaloTag, TEV cleavage site, and multiple-cloning-site, inserted from plasmid # DU23222 (James Hastie, MRC-PPU Reagents & Services, UK)
• Vector type: Bacterial Expression
• Promoter: T7
• Tags / Fusion Proteins:
  • HaloTag (N terminal on backbone)
  • TEV cleavage site (ENLYFQ/G) (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7
• 3′ sequencing primer: T7 Term

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Original plasmid obtained from Daniel Daley (Addgene #154464) and HaloTag-TEVc-empty sub-cloned in from a plasmid (Cat # DU23222) from MRC-PPU Reagents & Services, UK.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • HaloTag Vector Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please visit https://doi.org/10.64898/2026.02.13.703496 for bioRxiv preprint.