Ubi-SCAPE Enables Deep Exploration of the Poly-Ubiquitylome
Johnston HE, Frey AM, Barve G, Carling S, Trost M, Samant RS
J. Proteome Res. 2026
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Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 254152 | pET28a HaloTag-TEVc-empty | Backbone for bacterial expression with N-terminal HaloTag and a TEV-protease cleavage site |
| 254153 | pET28a HaloTag-TEVc-6xtrTUBE | Bacterial expression of 6xtrTUBE with an N-terminal HaloTag followed by a TEV protease cleavage site |
| 254154 | pET28a 6xHis-HaloTag-TEVc-4xtrTUBE | Bacterial expression of 4xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site |
| 254155 | pET28a 6xHis-HaloTag-TEVc-4xtrTUBEmut | Bacterial expression of binding-deficient 4xtrTUBEmut with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site |
| 254156 | pET28a 6xHis-HaloTag-TEVc-6xtrTUBE | Bacterial expression of 6xtrTUBE with an N-terminal 6xHis and HaloTag followed by a TEV protease cleavage site |