pSH3
(Plasmid
#254172)
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Purpose(Empty Backbone) Effector backbone for TF expression. Contains a CaMV 35S promoter and a C-terminal tag (6xMyc) with an MCS for gene insertion.
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254172 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonePZP200
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Vector typePlant Expression
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Tag
/ Fusion Protein
- 6xMyc (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Resource Information
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSH3 was a gift from Sun-Hwa Ha (Addgene plasmid # 254172 ; http://n2t.net/addgene:254172 ; RRID:Addgene_254172) -
For your References section:
tRUBY: A convenient in planta tool for the detection of protein-DNA and protein-protein interactions. Kim S, Choi H, Yi TG, Ha SH. Mol Cells. 2026 Apr 10:100357. doi: 10.1016/j.mocell.2026.100357. 10.1016/j.mocell.2026.100357 PubMed 41967643