tRUBY: A convenient in planta tool for the detection of protein-DNA and protein-protein interactions.

Kim S, Choi H, Yi TG, Ha SH
Mol Cells. 2026 Apr 10:100357. doi: 10.1016/j.mocell.2026.100357. PubMed Article

Plasmids from Article

ID	Plasmid	Purpose
254170	pSH1	Promoter-less backbone for PDI assays. Designed for inserting a target promoter into the MCS upstream of the tRUBY reporter.
254171	pSH2	Effector backbone for TF expression. Contains a CaMV 35S promoter and a C-terminal tag (3xHA) with an MCS for gene insertion.
254172	pSH3	Effector backbone for TF expression. Contains a CaMV 35S promoter and a C-terminal tag (6xMyc) with an MCS for gene insertion.
254173	pSH4	Effector backbone for TF expression. Contains a CaMV 35S promoter and a C-terminal tag (GST) with an MCS for gene insertion.
254177	pSH5	Universal reporter plasmid for PPI assays. Encodes a 4xUAS-driven tRUBY cassette for visual detection in the GAL4/UAS system.
254178	pSH6	PPI screening backbone. Contains a 35S promoter and VP16-AD (Prey) with various C-terminal epitope tags.
254179	pSH7	PPI screening backbone. Contains a 35S promoter and VP16-AD (Prey) with various C-terminal epitope tags.
254180	pSH8	PPI screening backbone. Contains a 35S promoter and VP16-AD (Prey) with various C-terminal epitope tags.
254181	pSH9	PPI screening backbone. Contains a 35S promoter and GAL4-BD (Bait) with various C-terminal epitope tags.
254182	pSH10	PPI screening backbone. Contains a 35S promoter and GAL4-BD (Bait) with various C-terminal epitope tags.
254183	pSH11	PPI screening backbone. Contains a 35S promoter and GAL4-BD (Bait) with various C-terminal epitope tags.