pSH9
(Plasmid
#254181)
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Purpose(Empty Backbone) PPI screening backbone. Contains a 35S promoter and GAL4-BD (Bait) with various C-terminal epitope tags.
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Depositing Lab
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Sequence Information
Ordering
This material is available to academics and nonprofits only.
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254181 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonePZP200
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Vector typePlant Expression
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Tags
/ Fusion Proteins
- GAL4BD (C terminal on insert)
- 3xHA (C terminal on insert)
Growth in Bacteria
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Bacterial Resistance(s)Spectinomycin, 50 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSH9 was a gift from Sun-Hwa Ha (Addgene plasmid # 254181 ; http://n2t.net/addgene:254181 ; RRID:Addgene_254181) -
For your References section:
tRUBY: A convenient in planta tool for the detection of protein-DNA and protein-protein interactions. Kim S, Choi H, Yi TG, Ha SH. Mol Cells. 2026 Apr 10:100357. doi: 10.1016/j.mocell.2026.100357. 10.1016/j.mocell.2026.100357 PubMed 41967643
Map uploaded by the depositor.
