pSH5
(Plasmid
#254177)
-
PurposeUniversal reporter plasmid for PPI assays. Encodes a 4xUAS-driven tRUBY cassette for visual detection in the GAL4/UAS system.
-
Depositing Lab
-
Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254177 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
-
Vector backbonePZP200
-
Vector typePlant Expression
Growth in Bacteria
-
Bacterial Resistance(s)Spectinomycin, 50 μg/mL
-
Growth Temperature37°C
-
Growth Strain(s)DH5alpha
-
Copy numberHigh Copy
Gene/Insert
-
Gene/Insert nametRUBY
-
Alt name4xUAS-min35S:tRUBY
-
Alt nameGAL4/UAS-responsive reporter
Terms and Licenses
-
Academic/Nonprofit Terms
-
Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
-
For your Materials & Methods section:
pSH5 was a gift from Sun-Hwa Ha (Addgene plasmid # 254177 ; http://n2t.net/addgene:254177 ; RRID:Addgene_254177) -
For your References section:
tRUBY: A convenient in planta tool for the detection of protein-DNA and protein-protein interactions. Kim S, Choi H, Yi TG, Ha SH. Mol Cells. 2026 Apr 10:100357. doi: 10.1016/j.mocell.2026.100357. 10.1016/j.mocell.2026.100357 PubMed 41967643