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pPF1653
(Plasmid #254186)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254186 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pQE-80L
  • Backbone size (bp) 3821
  • Modifications to backbone
    Addition of OriT/RP4 for conjugation, removal of ampicillin resistance, removal of 6xHis tag, T5 lac promoter and LacIq. Insertion of eYFP. Addition of transcriptional terminators.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPF1653 was a gift from Peter Fineran (Addgene plasmid # 254186 ; http://n2t.net/addgene:254186 ; RRID:Addgene_254186)

  • For your References section:

    SorTn-seq: a high-throughput functional genomics approach to discovering regulators of bacterial gene expression. Smith LM, Jackson SA, Gardner PP, Fineran PC. Nat Protoc. 2021 Sep;16(9):4382-4418. doi: 10.1038/s41596-021-00582-6. Epub 2021 Aug 4. 10.1038/s41596-021-00582-6 PubMed 34349283
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