SorTn-seq: a high-throughput functional genomics approach to discovering regulators of bacterial gene expression.

Smith LM, Jackson SA, Gardner PP, Fineran PC
Nat Protoc. 2021 Sep;16(9):4382-4418. doi: 10.1038/s41596-021-00582-6. Epub 2021 Aug 4. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID	Plasmid	Purpose
254184	pPF1439	Double reporter vector (mCherry under T5-lac promoter + promoterless eYFP), pBR322/OriV, RP4/OriT, CmR. Clone your promoter of interest in front of eYFP. Double reporter vector for flow cytometry.
254185	pPF1438	mCherry under T5-lac promoter (IPTG inducible), pBR322/OriV, RP4/OriT, CmR. mCherry only control for flow cytometry.
254186	pPF1653	eYFP (promoterless), pBR322/OriV, RP4/OriT, CmR. Clone your promoter of interest in front of eYFP. eYFP only control for flow cytometry.