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pKRCPN2
(Plasmid #254603)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254603 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    pDS1028
  • Backbone manufacturer
    Kevin Roberts and George Salmond, Cambridge University
  • Backbone size (bp) 5664
  • Modifications to backbone
    Swap of Tn-DS1028Cm with Tn-DS1028uidAKm
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Tetracycline, 5 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Pir1
  • Growth instructions
    Kanamycin resistance is encoded on transposon and should be used to select for mutants following transposition.
  • Copy number
    Low Copy

Cloning Information

  • Cloning method Restriction Enzyme

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pKRCPN2 was a gift from Peter Fineran (Addgene plasmid # 254603 ; http://n2t.net/addgene:254603 ; RRID:Addgene_254603)

  • For your References section:

    SorTn-seq: a high-throughput functional genomics approach to discovering regulators of bacterial gene expression. Smith LM, Jackson SA, Gardner PP, Fineran PC. Nat Protoc. 2021 Sep;16(9):4382-4418. doi: 10.1038/s41596-021-00582-6. Epub 2021 Aug 4. 10.1038/s41596-021-00582-6 PubMed 34349283
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