pLenti U6_r2ad_ IN-PE2-SSB_PuroR
(Plasmid
#254321)
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Purpose(Empty Backbone) A lentiviral vector expressing a modified version of PE2 with a prime editing gRNA cloning site. Peptide fusion (Velimirovic et al.) and fusion with the La protein (Yan et al.) enhance PE efficiency
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 254321 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $94 | |
Backbone
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Vector backbonepLenti U6_r2ad_ IN-PE2-SSB_PuroR
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Vector typeMammalian Expression, Lentiviral, CRISPR
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature30°C
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Growth Strain(s)NEB Stable
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameNone
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Please visit https://doi.org/10.64898/2025.12.16.694467 for bioRxiv preprint.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLenti U6_r2ad_ IN-PE2-SSB_PuroR was a gift from Richard Sherwood (Addgene plasmid # 254321 ; http://n2t.net/addgene:254321 ; RRID:Addgene_254321) -
For your References section:
LDLR variant classification through activity-normalized prime editing screening. Zhou PJ, Velimirovic M, Yu T, Gligorovski V, Mathis N, Zhao J, Phan QV, Vogd F, Ryu J, Pan Q, Tyagi A, Ascher DB, Schwank G, Pinello L, Cassa CA, Sherwood RI. bioRxiv [Preprint]. 2025 Dec 18:2025.12.16.694467. doi: 10.64898/2025.12.16.694467. 10.64898/2025.12.16.694467 PubMed 41473288