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lentiTet-LDLR-mCherryFus-D227E BlastR
(Plasmid #254334)

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This material is available to academics and nonprofits only.
Item Catalog # Description Quantity Price (USD)
Plasmid 254334 Standard format: Plasmid sent in bacteria as agar stab 1 $94
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Backbone

  • Vector backbone
    lentiTet-LDLR-mCherry BlastR
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    Blasticidin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    30°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    LDLR
  • Alt name
    FH, FHC, FHCL1, LDLCQ2
  • Species
    H. sapiens (human)
  • Mutation
    D227E
  • Entrez Gene
    LDLR (a.k.a. FH, FHC, FHCL1, LDLCQ2)
  • Tag / Fusion Protein
    • mCherry (C terminal on insert)

Cloning Information

  • Cloning method Gibson Cloning

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry

Trademarks:

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please visit https://doi.org/10.64898/2025.12.16.694467 for bioRxiv preprint.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    lentiTet-LDLR-mCherryFus-D227E BlastR was a gift from Richard Sherwood (Addgene plasmid # 254334 ; http://n2t.net/addgene:254334 ; RRID:Addgene_254334)

  • For your References section:

    LDLR variant classification through activity-normalized prime editing screening. Zhou PJ, Velimirovic M, Yu T, Gligorovski V, Mathis N, Zhao J, Phan QV, Vogd F, Ryu J, Pan Q, Tyagi A, Ascher DB, Schwank G, Pinello L, Cassa CA, Sherwood RI. bioRxiv [Preprint]. 2025 Dec 18:2025.12.16.694467. doi: 10.64898/2025.12.16.694467. 10.64898/2025.12.16.694467 PubMed 41473288
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